![]() ![]() Pansit-pansitan, scientifically known as Pepromia pellucida, is a medicinal plant valued for its anti-gout properties which helps lower uric acid in the blood. A single pansit-pansitan plant can be maintained in a 500 ml full-strength Hoagland's solution for seven months This means that one must replenish the medium with a fresh solution to encourage and maintain normal growth of pansit-pansitan. ![]() Decreasing the volume of full-strength Hoagland's solution reduced the values of all the above parameters. Results of further studies indicated that a decrease in the strength and yield of the Hoagland's solution decreased the shoot length, number of branches, fresh weight and dry weight of the plant. Pansit-pansitan plants grown hydroponically had lower Pb and Cd content than those grown in soil. On the other hand, plants grown in the UPLB greenhouse and geneback had Cd and Pb within the WHO limit. Metal analysis showed that pansit-pansitan grown and collected from various locations showed that none of the plant samples passed the WHO standard of Cd and Pb. To regulate the uptake of nutrient element in pansit-pansitan, the use of hydroponic culture through non-aerated Hoagland's solution was studied. It was reported that pansit-pansitan contained high amount of toxic metal like lead (Pb) and cadmium (Cd) surpassing the limits allowed by the WHO. ![]() It was one of the ten clinically tested medicinal plants endorsed by the Department of Health. Tsaang gubat and ampalaya aqueous leaf extracts did not exhibit any anti-amoeba activity.Pansit-pansitan, scientifically known as Pepromia pellucida, is a medicinal plant valued for its anti-gout properties which helps lower uric acid in the blood. Metronidazole was able to eradicate the amoeba parasite at 24 and 72 hours, while exposure to DMSO did not result in inhibition nor death of the parasite. The IC50 of tsaang gubat and ampalaya leaf extracts were >500 µg/mL at 24, 48, and 72 hours. Tsaang gubat and ampalaya leaves failed to show anti-amoebic activity and even had increased growth of amoeba at all dose levels. ![]() Metronidazole and DMSO served as positive and negative controls, respectively. The live trophozoites (unstained) were counted under a binocular microscope. The trophozoites were stained with Trypan blue and dispensed into chambers of a Neubauer hemocytometer. The amoeba and leaf extracts were incubated for 24, 48, and 72 hours. Anti-amoebic activity was determined in an in vitro assay using Entamoeba histolytica HK-9 strain against 10 dose levels (18-10,000 µg/mL). The tsaang gubat and ampalaya leaves were sourced from the University of the Philippines at Los Baños and processed into a lyophilized aqueous extract. The aim of this research was to provide evidence to validate the use of tsaang gubat and ampalaya leaf extracts for dysentery by determining their anti-amoebic activity. Tsaang gubat and ampalaya have been used by traditional healers from different cultures to treat dysentery. Few drugs such as metronidazole are available to treat this disease unfortunately, it is associated with several serious side effects. Amoebiasis is a global health problem affecting poor regions in the world. ![]()
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